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. 2013 Aug 28;33(35):14001–14016. doi: 10.1523/JNEUROSCI.1307-13.2013

Figure 8.

Figure 8.

Planar polarity refinement does not occur in the utricular maculae. A, Schematic illustrating the positions of the three analysis fields relative to the position of the LPR. Hair cells in the maculae are patterned about an LPR, and, as a result, cells in the LES have stereociliary bundle orientations that are opposite of those in the striola and MES. B, Individual stereociliary bundle orientations for vestibular hair cells of the utricular maculae at P0 or between P10 and P12 graphed as circular histograms demonstrate stereociliary bundle misorientation in the striola region of the Vangl2 CKO. Unlike the case for auditory hair cells, the orientation of affected cells in the striola of Vangl2 CKO mice is not refined during the first 12 postnatal days. For these histograms, 90° is directed toward the lateral and 180° toward the medial edge of the utricular maculae, and each bin is 12°. The total number of hair cells represented by each histogram (n) is shown, and black bars mark the mean stereociliary bundle orientation. C, Averaged vestibular stereociliary bundle deviations from a polarity reference drawn perpendicular to the LPR. Error bars show SD, and asterisks indicate statistical significance for differences between Vangl2 CKOs and littermate controls of the same hair cell type and utricular position calculated using two-tailed Student's t test with unequal variance (*p < 0.025). D, The CV of stereociliary bundle orientation for hair cells located in each vestibular analysis field demonstrates the loss of coordinated orientation for hair cells located in the striola region. In this analysis, a CV value of 0 occurs for uniform bundle orientations, and each symbol represents a single analysis field. No difference in the distribution of CV values occurs between P0 and P12, indicating that active refinement does not occur in the vestibular maculae. The number of mice assayed at P0 is n = 4 for WT and n = 5 CKO, and, at P10–P12, n = 6 for WT and n = 6 for CKO. LES, Lateral extra striolar region; MES, medial extra striolar region.