Figure 4.
TYRA-3 couples to Gαq and both Gαq and Gαs signaling in the ASIs are required for TA inhibition. A, B, TA stimulates phosphoinositide metabolism in COS-7 cells expressing TYRA-3a. COS-7 cells transiently transfected with TYRA-3a–pFLAG were labeled with [3H]inositol and incubated with various monoamines at [10 μm] (A) or TA at a range of concentrations (B). PI turnover was assayed according to Tejada et al. (2006). Values are expressed as percentage stimulation above basal. C, Wild-type and transgenic animals expressing cell-specific RNAi were assayed for aversive responses to 30% 1-octanol with 5-HT ± TA. Black bars, Wild-type animals; red bars, RNAi in wild-type animals. Data are presented as mean ± SE and analyzed by two-tailed Student's t test. *p < 0.001, significantly different from wild-type animals examined under identical conditions.