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Regions of interest used for magnetic resonance spectroscopy (MRS) centered on the anterior cingulate (AC) gyrus (A) and centrum semiovale (CSO) white matter (B). The AC voxel size was 3.5 × 3.5 × 3.5 cm3, whereas the CSO voxel was 5.0 × 3.0 × 3.0cm3. Example MEGA-PRESS spectra for gamma-aminobutyric acid (GABA) (C, D) and N-acetyl-aspartyl-glutamate (NAAG) (E, F), as well as the conventional short TE PRESS spectrum (G, H), corresponding to each region are shown.
