Figure 8.

Neuropeptide Y (NPY)-immunopositive interneurons in the rat perirhinal cortex. Photomicrographs showing interneurons stained with an antibody against NPY in the perirhinal cortex of 3-week-old rats (A–C). Specifically, NPY immunostaining is shown in a control, non-epileptic rat (A) and in pilocarpine-treated rats 3 (B) and 14 days (C) after pilocarpine treatment. (D) Normalized (respect to control) quantification of NPY immunostained neurons in 3 and 8-week-old rats following pilocarpine treatment. Note in the 3-week-old animals (n = 3–6 for each time interval) that NPY immunostained neurons increased significantly 1 and 3 days after pilocarpine, but recovered 7 and 14 days later. Note that a consistent decrease in NPY interneurons was instead observed in 8-week-old rats (n = 4–5 for each time interval). ^**p < 0.01, analysis of variance followed by Tukey's test for multiple comparisons. Scale bar: 100 μm. Animal treatment is described in Biagini et al. (2008). Details of the immunostaining procedure and cell counts are as in Bortel et al. (2010); Benini et al. (2011) and Gualtieri et al. (2013).