Fig. 11.

Thin-layer chromatogram of lipids in the Harderian glands of golden hamsters.

Golden hamsters were injected with 2.5 μCi of [U-¹⁴C]leucine into peritoneum. Lipids in the gland were obtained and developed on a TLC plate. A, a representative autoradiogram of a plate. B, H₂SO₄ detection of lipids on the same plate in panel A. Lipids obtained at 1 h after injection were spotted on lanes 1 and 4, at 3 h on lanes 2 and 5, and at 6 h on lanes 3 and 6. Lanes 1 to 3 contained male lipids, while lanes 4 to 6 contained female lipids. “ADG” and “FFA” indicate the positions of 1-alkyl-2,3-diacylglycerol and free fatty acids, respectively.