Fig. 15.

Northern blot hybridization analysis of IVD mRNA contents in the Harderian glands of golden hamsters and effect of testosterone enanthate (TE) on their expression.

Total RNA was prepared from Harderian glands of golden hamsters and mRNA of IVD was measured by Northern blot hybridization analysis. A 30 μg portion of total RNA was put on each lane. The positions of ribosomal RNA are indicated on the right. Bands of ribosomal RNA stained by ethidium bromide (bottom, the sizes shown on the right) demonstrate equal amounts of total RNA par lane. The blot shows a typical result of a series of experiments. Lanes 1 and 2, male; lanes 3 and 4, castrated male; lanes 5 and 6, castrated male treated with 5 mg/kg TE, lanes 7 and 8, female; lanes 9 and 10, female treated with 5 mg/kg TE. TE, testosterone enanthate.