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. 2007 May;83(3):77–96. doi: 10.2183/pjab.83.77

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© 2007 The Japan Academy

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 17. — Effect of androgen on the expression of IVD and androgen receptor (AR) in the Harderian gland of golden hamsters. Golden hamsters (8 weeks old) were injected subcutaneously with 5 mg/kg of testosterone enanthate and Harderian glands were obtained after indicated time. Total RNA was extracted from them and mRNA was amplified by reverse transcription-polymerase chain reaction (RT-PCR) with specific primers for IVD, androgen receptor (AR), and beta-actin. Representative ethidium bromide-stained gels are shown. Castration of male hamsters was done 2 weeks before injection. Lane 1, male; lanes 2 to 4, castrated male; lane 2, 0 day; lane 3, 3 days, lane 4, 7 days after injection.