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. 2007 May;83(3):77–96. doi: 10.2183/pjab.83.77

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© 2007 The Japan Academy

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 8. — Radio-GLC analysis of fatty acid methyl esters prepared from ADG of golden hamster Harderian glands.

Golden hamsters were injected with 25 μCi of [U-¹⁴C]leucine and glands were obtained at 6 h after injection. Lipids in the glands were obtained and ADG was eluted. The acyl groups of ADG were extracted as fatty acid methyl esters. They were dissolved in hexane and then analyzed with a Gas Chromatograph GC-6AM (Shimadzu). The column effluent was passed through an Aloka peak analyzer radio-gas chromatograph system (Aloka). The voltage of the flame ionization detector (FID) was plotted upward and radio-activity was plotted downward. A, male; B, female. Each number indicates the chain length of each peak. “br” means branched chain.