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. Author manuscript; available in PMC: 2013 Aug 29.
Published in final edited form as: Cell. 2011 Apr 29;145(3):447–458. doi: 10.1016/j.cell.2011.03.032

Figure 4. Time-Lapse Live-Cell Imaging of Xic Pairing Events in Early Differentiating ES Cells.

Figure 4

(A) Representative snapshots of a day 1 differentiated PGKT2-TetR cell imaged over 30 min period at 1 min time intervals. Note that the two XicTetO loci show complete overlap at the 21 min time point. Open circles: Xic pairing (d ≤ 2 μm). Scale bar = 10 μm. The whole movie is available as Movie S4.

(B) A representative image of a day 1 differentiated PGKT2-TetR cell imaged over 150 min at 5 min intervals. Note that the two XicTetO loci show pairing (≤2 μm, open circle) for 40 min (from the 55 min to the 95 min time points), and they show closest proximity at the 75 min time point. Scale bar = 10 μm. See also Movie S5.