FIGURE 1:

Overexpression of Net1 isoforms causes nuclear morphology defects. (A) HeLa cells were transfected with HA-Net1, HA-Net1A, or HA-Net1ΔN. Two days later the cells were fixed and stained for HA-epitope proteins (green), α-tubulin (red), and DNA (blue). Shown are representative micrographs. Bar, 10 μm. (B) Functional domains in Net1 proteins. DH, Dbl homology domain; PH, pleckstrin homology domain; orange ovals, nuclear localization signal sequences; purple ovals, C-terminal PDZ domain–binding site; blue bar, Net1-specific sequence (amino acids 1–85); green bar, Net1A-specific sequence (amino acids 1–31); numbers refer to amino acids for mouse Net1 proteins. (C) Quantification of defects in nuclear morphology. Average of three independent experiments. Errors are SEM. Statistical significance compared with control values was determined by Student's t test; *p < 0.05; **p < 0.01; ***p < 0.001.