FIGURE 6:

Depletion of RhoA or RhoB does not phenocopy the effects of Net1 knockdown on nuclear morphology. (A) HeLa cells were transfected with control, RhoA, RhoB, or Net1-specific siRNAs. Two days later the cells were fixed and stained for α-tubulin (green), CREST (red), and DNA (blue). Representative micrographs. Bar, 10 μm. (B) Quantification of aberrant nuclear morphologies in siRNA-transfected cells. Average of three independent experiments. Errors are SEM. Statistical significance compared with control values was determined by Student's t test; *p < 0.05; **p < 0.01. (C) Representative Western blot of siRNA-transfected cells. (D) RhoA activity in asynchronous and prometaphase-arrested cells transfected with control or Net1 specific siRNAs. Average of three independent experiments. Errors are SEM.