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. Author manuscript; available in PMC: 2013 Nov 10.
Published in final edited form as: Nature. 2013 May 1;497(7448):211–216. doi: 10.1038/nature12143

Figure 6. Central and systemic actions of GnRH in counteracting aging.

Figure 6

a–d C57BL/6 mice at an old age were subjected to neurogenesis (a&b) and survival (c&d) assays, as detailed in methods. a: BrdU staining of MBH and dentate gyrus (DG) in neurogenesis assay. Bar = 50 µm. b&c: BrdU-labeled cells (BrdU+) in the MBH (b) and DG (c) in neurogenesis assay. d&e: Survival of BrdU-labeled (BrdU+) cells in the MBH (d) and DG (e) in survival assay. f–i. MBH-IKKβ and MBH-Con mice at an old age were daily injected subcutaneously with GnRH vs. vehicle for 5 weeks, and analyzed for muscle endurance (f), skeletal muscle fibers(g), skin (h), and cognition (i) (see suppl. Fig. 10 for additional data). *P < 0.05, **P < 0.01, ***P < 0.001; n = 4 (b–e), 7 (f), and 3 (g&h) per group, and n = 12 (Con, Veh), 7 (Con, GnRH), 7 (IKKβ, Veh), and 8 (IKKβ, GnRH) (i). Error bars reflect mean ± SEM.