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. 2013 Aug 29;8(8):e73206. doi: 10.1371/journal.pone.0073206

Figure 2. Exposure to IR abrogates adipogenesis independently of the stromal lineage potential.

Figure 2

(A) MSC and 3T3-L1 cell populations were exposed (IR) or not (CTRL) to 10 Gy IR and one week later placed in adipogenic differentiation media. Representative photographs showing lipid accumulation stained with Oil Red O is shown for each population. (B) Quantification of lipid accumulation was determined by the extraction of Oil Red O staining and detection by spectrophotometry. (C–D) Expression of PPARγ was determined by quantitative real-time PCR using RNA extracted from control and IR-induced senescent MSC and 3T3-L1 populations cultured or not in adipogenic differentiation media. Mean ± standard error of at least three individual experiments is shown. p values were obtained by performing a Student’s t-test. *: p value < 0.05.