Table 1. Details of specific primers used in Real-Time PCR experiments.
| GENE | PRIMER SEQUENCE | GenBank, accession # | AMPLICON bp | Ta, °C | EFFICIENCY, % | R2 |
| Sdha | F: CTCTTTTGGACCTTGTCGTCTTT | NM_130428 | 102 | 60 | 104.7 | 0.999 |
|
R: TCTCCAGCATTTGCCTTAATCGG |
|
|
|
|
|
| Hprt1 | F: CCCAGCGTCGTGATTAGTGATG | NM_012583 | 125 | 60 | 103 | 0.998 |
|
R: TTCAGTCCTGTCCATAATCAGTC | |||||
| Tbp | F: CACCGTGAATCTTGGCTGTAAAC | NM_001004198 | 123 | 60 | 105 | 0.998 |
|
R: CGCAGTTGTTCGTGGCTCTC |
|
|
|
|
|
| ANP | F: AAGTGTAGATGAGTGGTT | NM_012612 | 118 | 58 | 95.3 | 0.999 |
|
R: TGGTGCTGAAGTTTATTC |
|
|
|
|
|
| BNP | F: ATCTGTCGCCGCTGGGAGGT | NM_031545 | 187 | 60 | 101.1 | 0.999 |
|
R: TGGATCCGGAAGGCGCTGTCT |
|
|
|
|
|
| CNP | F: GGAGCCAATCTCAAGGGA | NM_053750 | 201 | 60 | 103.9 | 0.997 |
|
R: TGCCGCCTTTGTATTTGC |
|
|
|
|
|
| NPR-A | F: AGAGCCTGATAATCCTGAGTA | NM_012613 | 81 | 58 | 95.4 | 0.998 |
|
R: A TCCACGGTGAAGTTGAAC |
|
|
|
|
|
| NPR-B | F: CCCATCCTGTGATAAAACTCC | NM_053838 | 89 | 60 | 104 | 0.999 |
|
R: AAGCTGGAAACACCAAACA |
|
|
|
|
|
| NPR-C | F: GGACCGCGAAGCCTGAGTTTGAGA | NM_012868 | 240 | 64 | 100.3 | 0.998 |
|
R: ATGGACACCTGCCCGGCGATACCT |
|
|
|
|
|
| IL-6 | F: ACCACCCACAACAGACCAGT | NM_012589 | 141 | 60 | 96 | 0.997 |
|
R: ACAGTGCATCATTCGCTGTTC |
|
|
|
|
|
| TNF-α | F: GCCCAGACCCTCACACTC | NM_012675 | 98 | 60 | 105 | 0.997 |
|
R: CCACTCCAGCTGCTCCTCT |
|
|
|
|
|
Legend: Sdha: Succinate dehydrogenase complex, subunit A, flavoprotein; Hprt1: Hypoxanthine phosphoribosyltransferase 1; Tbp: TATA binding protein; ANP: atrial natriuretic peptide; BNP: B-type (or brain) natriuretic peptide; CNP: C-type natriuretic peptide; NPR-A: Natriuretic peptide receptor A; NPR-B: Natriuretic peptide receptor B; NPR-C: Natriuretic peptide receptor C or clearance receptor; IL-6: Interleukin-6; TNF-α: Tumor necrosis factor-alpha.