Figure 7. Sequence conservation of cleavage sites in orthologs of EpCAM found in fishes, amphibians, birds, to placental mammals.
Amino acid sequences of 52 orthologs of human EpCAM were aligned using ClustallW and sequence conservation of each amino acid was calculated (maximum score 11. Shown are the mean conservation score throughout all orthologs (mean) and conservation scores of single amino acids ranging positions p−3 to p+3 around determined cleavage sites of metalloproteases (A), BACE1 (B), γ-cleavage of γ-secretase (C), and ε-cleavage of γ-secretase (D).