Table 1. Primers and probes sequences used for qPCR assays in this study.
| Primers Probes | Sequences 5′- 3′ | Modifications | Size (bp) |
| PLU F | GCTCTTTCTTGATTTCTTGGATG | ||
| PLU R | AGCAGGTTAAGATCTCGTTCG | ||
| PLU P | ATGGCCGTTTTTAGTTCGTG | CY5-IB | 100 |
| FAL F | ATTGCTTTTGAGAGGTTTTGTTACTTT | ||
| FAL R | GCTGTAGTATTCAAACACAATGAACTCAA | ||
| FAL P | CATAACAGACGGGTAGTCAT | FAM-MGB | 95 |
| VIV F | GCAACGCTTCTAGCTTAATCCAC | ||
| VIV R | CAAGCCGAAGCAAAGAAAGTCC | ||
| VIV P | ACTTTGTGCGCATTTTGCTA | VIC-MGB | 133 |
| RNaseP F | TGTTTGCAGATTTGGACCTGC | ||
| RNaseP R | AATAGCCAAGGTGAGCGGCT | ||
| RNaseP P | TGCGCGGACTTGTGGA | NED-MGB | 84 |
| IC F | AAAGAAACTAGGAGAGATGTGGAACAA | ||
| IC R | AGCTTGGCAGCTTTCTTCTCA | ||
| IC P | ACTGCAGCAGATGACAAGCAGCCCT | CY3-IB | 75 |
Primer and probes for amplification of Plasmodium spp., P. falciparum, P. vivax, RNaseP and internal control (IC) plasmid DNA assays. Sequences for Forward (F), Reverse(R) primers and the Probe (P) are shown.