Table 2. Analysis of the multiplex real-time PCR assay.
| Sample Name | Assay Performed | Analysis 1 Mean CT | Analysis 2 Mean CT | Analysis 3 Mean CT |
| DNA dilution # 1 | PLU | 19.8 | 19.85 | 19.92 |
| DNA dilution # 2 | PLU | 22.08 | 22.13 | 22.01 |
| DNA dilution # 3 | PLU | 24.38 | 24.43 | 24.33 |
| DNA dilution # 4 | PLU | 26.73 | 26.78 | 26.79 |
| DNA dilution # 1 | FAL | 21.93 | 21.77 | 22.08 |
| DNA dilution # 2 | FAL | 24.28 | 24.19 | 24.6 |
| DNA dilution # 3 | FAL | 26.32 | 26.3 | 27.01 |
| DNA dilution # 4 | FAL | 29.62 | 29.49 | 30.06 |
| DNA dilution # 1 | VIV | 22.17 | 22 | 22.22 |
| DNA dilution # 2 | VIV | 24.9 | 24.63 | 24.96 |
| DNA dilution # 3 | VIV | 27.39 | 27.08 | 27.46 |
| DNA dilution # 4 | VIV | 30.27 | 29.94 | 30.05 |
| DNA dilution # 1 | RNaseP | 25.81 | 22.5 | 25.24 |
| DNA dilution # 2 | RNaseP | 27.79 | 24.98 | 27.62 |
| DNA dilution # 3 | RNaseP | 29.98 | 27.38 | 30.29 |
| DNA dilution # 4 | RNaseP | 33.92 | 30.31 | 32.75 |
Multiplex qPCR assays were performed containing both primer and probe sets for all four targets or primer and probe set for single target. Analysis 1 shows data from multiplex assay analyzed as multiplex where all four targets were analyzed simultaneously. Analysis 2 shows data from multiplex assay but data was analyzed as a single assay for each target. Analysis 3 shows data from single assays.