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. 2013 Aug 29;8(8):e71539. doi: 10.1371/journal.pone.0071539

Table 3. Inhibition studies to test the co-purification of PCR inhibitors.

ME High ME Low EZ High EZ Low Control High Control Low
Mean CT 23.4 28.86 23.65 28.92 23.32 28.5
STDEV 0.293 0.071 0.111 0.074 0.265 0.141

Two different concentrations of IC DNA were used as the DNA template in the qPCR reactions to test the co-purification of PCR inhibitors in samples extracted from whole blood using ME or EZ methods; High DNA concentration or Low DNA concentration. Control experiments did not contain genomic DNA in the reaction. Each column shows the method which the genomic DNA present [or not for the controls] was extracted [ME or EZ] and amount of IC plasmid present [High or Low].