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. 2013 Aug 29;8(8):e74108. doi: 10.1371/journal.pone.0074108

Figure 3. Effects of SM934 on the polarization of CD4+ T cells in EAE mice.

Figure 3

Splenocytes from immunized mice treated with vehicle or SM934 were isolated at the peak of disease (day 18 p.i.) and analyzed. (A) The percentage of Th1, Th17 and Treg cells in the CD4+ gate were analyzed by flow cytometry, values in the bar graphs are the mean ± SEM (n = 4). (B) Splenocytes were re-stimulated with IL-12 (20 ng/ml) or IL-23 (20 ng/ml) for 72 hours, and supernatants were collected to measure IFN-γ and IL-17 levels, respectively. (C) RNA from splenocytes was analyzed by real-time PCR for expression of T-bet, RORγt and Foxp3. Results were expressed as mean ± SEM. *, p<0.05 compared with vehicle control. Three independent experiments were performed with similar results.