Figure 5. SM934 treatment enhanced Treg cells expansion in EAE mice.

(A) Vehicle and SM934 treated mice were given daily i.p. injection of BrdU for 3 days from day 17 p.i., splenocytes and MNC in spinal cord were isolated and analyzed for BrdU incorporation. The proliferative profile was evaluated in CD4⁺Foxp3⁻, CD4⁺Foxp3⁺ splenocytes, and CD4⁺Foxp3⁻, CD4⁺Foxp3⁺ from spinal cord. (B) Percentage of CD4⁺Foxp3⁻, CD4⁺Foxp3⁺ splenocytes, and CD4⁺Foxp3⁻, CD4⁺Foxp3⁺ from spinal cord are expressed as mean ± SEM (n = 4). (C) CD4⁺ T cells were purified by immunomagnetic negative selection from splenocytes, indicated genes expression was analyzed by real-time PCR. Results were expressed as mean ± SEM. *, p<0.05 compared with vehicle control. Three independent experiments were performed with similar results.