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. 2013 Aug 29;8(8):e72685. doi: 10.1371/journal.pone.0072685

Figure 2. Effects of KRG on pancreatic islet morphology and cell area in CsA-induced pancreatic injury.

Figure 2

(A) Double immunostaining for insulin (red) and glucagon (green) showing pancreatic islet morphology and size in the experimental groups. Well-preserved pancreatic islet structure and insulin-immunoreactive areas were seen in the groups treated with VH alone and with VH plus KRG. CsA treatment reduced the insulin-positive area and caused marked cellular vacuolization. However, CsA plus K0.2 or K0.4 treatments resulted in increased insulin immunoreactivity with less pronounced vacuolization, but the latter pathology was still present. (B) Quantitative analysis of total islet area evaluated by counting the insulin-positive areas lacking vacuolization per square millimeter of the pancreatic tissues in the experimental groups. The CsA plus K0.2 or K0.4 groups showed significantly increased insulin-positive areas compared with the CsA-only group. Values are means ± SE (n = 10). Magnifications×400. #P<0.05 vs. VH; *P<0.05 vs. CsA.