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. 2013 Aug 29;8(8):e72550. doi: 10.1371/journal.pone.0072550

Figure 5. Complementation of the fliH mutant with pBhSV2::pflgB-fliH restores the wild-type morphology and motility.

Figure 5

(A) Darkfield microscopy analysis of B. hermsii wild-type (WT) (i), fliH mutant (ii), fliH mutant transformed with empty vector pBhSV2 (iii) and fliH mutant complemented with pBhSV2::pflgB-fliH (iv). (B) Representative images of quantitative swimming plate assays show that the wild-type B. hermsii and fliH complemented mutant pBhSV2::pflgB-fliH swam equally whereas the fliH mutant swam significantly less (B & C). Results are the average of 3 independent biological experiments. The asterisk indicates that the swim diameter of the mutant was significantly less than those of the WT and complemented spirochetes (p<.05). Scale bars represent 10 µm.