Skip to main content
. 2013 Aug 29;8(8):e72550. doi: 10.1371/journal.pone.0072550

Figure 6. Comparison of flaB gene transcription and synthesis of flagellar proteins in B. hermsii wild-type (WT) and fliH mutant.

Figure 6

(A) Fold change of flaB transcript normalized to glpQ transcript was obtained from 3 individual cultures of WT and fliH mutant spirochetes. (B) Relative amounts of FlaB protein were normalized to GlpQ protein measured by semi-quantitative immunoblot using 3 lysates of the WT and mutant B. hermsii. (C) Immunoblot analysis of purified periplasmic flagella from wild-type B. hermsii (lanes a), lysates of wild-type B. hermsii (lanes b) and the fliH mutant (lanes c) probed with anti-FlaA antibody (left panel) and anti- FlaB (H9724) antibody (right panel). Molecular mass standards are shown on the left in kDa. (D) Comparative anti-FlgE and anti-FliI immunoblot analyses of WT, fliH mutant, fliH mutant pBhSV2 and fliH mutant pBhSV2:: pflgB-fliH spirochetes.