Table 1. Primers for sequencing TBX-1, TBX-5, GATA-4, GATA-6, SOX7, Nkx2.5 and Fog2.
Gene | Primer-F (5′-3′) | Primer-R (5′-3′) |
TBX1-1 | GGAGGAGCAGATGTCTCAGC | CCGGCTGCCTATACTCACTC |
TBX1-2 | CCATGACGCCATAATCCTCT | TTGTGTTTTCTCCCCTTTGC |
TBX1-3 | ACGCAGCTCTCGCATTTCT | GGCGGAGGATAGGTGTTAGG |
TBX1-4 | GCCAAGCTCCCAGTTGAGTA | TCGCAGGTGCCTAAAGAGTT |
TBX1-5 | GCAGCAGAGGGTTCAATCTC | TAGCCTCGCAGGGACTCTAA |
TBX1-6 | AGTGACCCAGCCTCATCTTG | GTCTAAGCGGACCCACTGTC |
TBX1-78 | CTTGGTGCGCTTCTCCTAAC | AGAGGGCCGAGGAGTGAG |
TBX1-9A | GTTGGGAGATGCAGTCCTGT | TACTGGACAGCAGCACTTGG |
TBX1-9B | GATGGTGTGTGAGGCTGATG | CTTGCATGCACACTTGACCT |
TBX1-9C | GGCCAAGAGCCTTCTCTCC | ACTGGGGAACCGGATACTTC |
TBX5-1 | CACTGAGTTATCGCATCCC | CACGAAGCCATTCTTATTT |
TBX5-2 | GTGGGAGCTAGTTGGATAGGC | CGAACAAGATGCGGTTTGAC |
TBX5-3 | CAAACTGCTCCCTCCTGT | AAGTAGATGGCAATACGCTA |
TBX5-4 | ACTGTGGGTTCAAGTGGT | AGGATCTATCTTTCGCTCT |
TBX5-5 | CCGCTTCCACGTTTCTCCAGG | TCTGAGCCTCCGCTTTCTCATCT |
TBX5-6 | CTCACCTGGTGCGTGAACTGAA | GGTAGAGGCAGAAAGCGACGAAAG |
TBX5-7 | AATGAAATCCCTGGCCCCTTTT | TTGTCCCCACCCCAGCACC |
TBX5-8 | GAAGTGGTGGGTCCCGTTGA | TGGAGGGAGGTGCTGGGTTG |
TBX5-9 | CTGGTTCAGCCACTCAGGAAATCT | CTCCAGCCTGGGTGATAGAGCA |
TBX5-10A | TTGTATTCAGAATGGCGGTTAGGG | AAGTGAGCGGAGAAGTGCTGGTAG |
TBX5-10B | TGCCCAGCCTAGAGGACATCAG | GGGGAGTAGCGTGAATGTGGC |
TBX5-10C | CAAGGTCGCTGGATGCT | TTCGGCTTTCAGTAAACA |
TBX5-10D | CCAACCTTCCAAACCTCCATCA | ACAACCTCTTCCTGTTTCCTCCAA |
GATA4-1 | GACTCCCACAGGCCAGTCAG | GACAAGCAAAGGCGGAGAAG |
GATA4-2 | ATTTGAAGCGTGGAAGAAGCAAC | CCTCGACAGGGCTCAAGACG |
GATA4-3 | TTGTTTCTGTGCGCTCTAG | TCTCACCCACGTAATCCC |
GATA4-4 | GAGTTAGGTGCCGTCACAGG | GGAAGAGGCCAGCAAAGTAG |
GATA4-5 | CTTAGGTGTTGCCTTCTCG | TTTGCTGGGCTCTTCATC |
GATA4-6 | GTTTGTCCCTGCCGCTGAT | GCTGCAAGTCCCACCCAGTA |
GATA4-7A | GGTCATAGCCCTGGTTGTAT | AGGCTGTGCTGTGGTGG |
GATA4-7B | CTGCATCCCTAATACCAAATC | AACCTCCCAGTGAAGACCA |
GATA6-1 | CCGTCCCCTCCCCACCCTCTTT | GAGATCGCGCGAGGAGGAAGCA |
GATA6-2A | TGGAGGCGAGGTAGCGTGCAG | AACTGAGCAGCAGCGAGCGGG |
GATA6-2B | CTGAGCCCCTTCGCACCCGAG | CCTAGGGCGGGCTGGGAGAGT |
GATA6-2C | CACCTGCAGGGGTCGGGCAGT | AAACAGGGCCCGAGTGGAGCA |
GATA6-3 | CTACTGGGGCGCTCCGGGTGT | AGCGGGTGGGCGTTGGAACAG |
GATA6-4 | TGGAGAAGAAACCAGGGATGA | TGCATTCAAATTTTTCACTTGAG |
GATA6-56 | CGGCCGCCAAATTCTTTTA | AACCATAAAAAAATGATACCGATCT |
GATA6-7 | TGGCCAGGGTCAGGTCAGTGG | GAGTGGCCCAAGCGCCCAGTT |
SOX7-1 | CCGCTCTGAATCCTGGGCACC | ACTCCCTCCCTCCGTCCTCCTCC |
SOX7-2A | AGTTAGCCATACTGGTTAATTTCTC | CCTCAGTGGGCATGTTCC |
SOX7-2B | GACGGCTCCTCTGCCACTCA | CCATCTCCTGCCTATTACTCCC |
SOX7-2C | CTGTGGGACCCGTTGGTGT | CATGGCCTCCTCTGCCTTGT |
NKX2.5-1 | GTGACACGAAACTGCTCATC | ACAACACCAGGCATCTTACA |
NKX2.5-2A | AAGTCACCGTCTGTCTCCCTC | GCTCTGAACCGCATTCAAGTC |
NKX2.5-2B | AAGCGCCGCAAGCTGAA | GGCCTCAATCCCTACGGTT |
FOG2-1 | TCATCTCCGAACGTGAATCCG | TGGGCAATAATCCCACCAACTC |
FOG2-2 | CGGATGTGGCATTATCT | TTACTCATGTCCCTCGA |
FOG2-3 | GAGGGTGTGAATGTGAAAGAG | CAAGCAGAGGTAGCACTTTGG |
FOG2-4 | GAGGTGGCTGCTGATAAAGTAC | GTTTCTGTCTAAATTCTGCGTAT |
FOG2-5 | GGTTTGGGAGATTTAGTTG | AAGATATTAGTCAAGCCACTC |
FOG2-6 | CATGAGAAGGTGCTATGGAC | GATGACGAGTTAGTGGGTG |
FOG2-7 | AATGGACAGCAGCAAAT | CTGGAGCAACAGAAGAAAC |
FOG2-8A | GAAAAGGTCCCTGTCATTC | CAGGTAGGCACATCTCATAC |
FOG2-8B | CTACACGCCACGACCCT | CATCTTGTTTCAGTCCACC |
FOG2-8C | GCTTCCTCAAATGGGTGT | CAGAGCCTGATTATCCAAGA |
FOG2-8D | GTCACAATACAGAAAAGCAT | GGTGCCATTTGGAAACTA |