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. 2013 Aug 29;9(8):e1003567. doi: 10.1371/journal.ppat.1003567

Figure 1. Schematic representation of the pBbIVET vector.

Figure 1

Features of this vector include: 3XTT, the transcriptional terminator sequence for bmpB [22] repeated in triplicate; pncA, promoterless pncA gene; flgB p kan, kanamycin resistance cassette; zeo, zeocin resistance marker; ColE1, E. coli origin of replication; ORFs 1, 2, 3, B. burgdorferi cp9 replication machinery. The EcoRI restriction site was used to clone the B. burgdorferi control in vivo-expressed promoter, ospC p, as well as the B. burgdorferi (Bb) gDNA library, in front of the promoterless pncA gene. The pBbIVET vector was derived from the B. burgdorferi shuttle vector pBSV2* [80].