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. 2013 Aug 29;9(8):e1003567. doi: 10.1371/journal.ppat.1003567

Figure 6. BBK46 protein production is detectable in E. coli but not in B. burgdorferi.

Figure 6

Immunoblot analysis of total protein lysate prepared from 1.5×108 B. burgdorferi Δbbk46 (Bb) or E. coli harboring either pBSV2G flaB p-bbk46-FLAG-cMyc (flaB p) or pBSV2G bbk46 p-bbk46-FLAG-cMyc (bbk46 p). Protein lysates were separated by SDS-PAGE and immunoblots performed using anti-FLAG monoclonal antibodies (α FLAG) and anti-cMyc monoclonal antibodies (α cMyc). 300 ng of purified PncA-FLAG [23] and GST-BmpA-cMyc [77] proteins served as positive controls (+) for each antibody. The positions of markers to the left of the panel depict protein standard molecular masses in kilodaltons.