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. 2013 Aug 29;9(8):e1003567. doi: 10.1371/journal.ppat.1003567

Figure 7. Spirochetes lacking bbk46 retain seroreactivity in mice.

Figure 7

Immunoblot analysis of sera collected three weeks post inoculation from groups of five C3H/HeN mice inoculated with clone A3-68ΔBBE02 (WT), bbk46::flaB p-aadA/pBSV2G (Δbbk46/vector) and bbk46::flaB p-aadA/pBSV2G-bbk46 (Δbbk46/bbk46+) at a dose of 1×104 spirochetes per mouse. (A) Total protein lysate from B. burgdorferi clone B31 A3 was probed with the serum from each individual mouse (1–5). (B) Purified recombinant GST-OspC protein was probed with pooled sera from the five mice in each infection group or αOspC polyclonal antibodies. The positions of markers to the left of the panel depict protein standard molecular masses in kilodaltons.