Table 2. The B. burgdorferi IVET system selects for in vivo-active promoters.
Number of BbIVET clones screened | Positive reisolation of infectious spirochetes from mouse tissuesa | Number of unique genomic fragments recovered | |||
Ear | Heart | Bladder | Joint | ||
∼15,000 | 175/179 | 173/179 | 172/179 | 174/179 | 289 |
Number of mice positive for spirochete reisolation/number of mice analyzed. Four mice were reisolate-negative for all tissues analyzed. Three mice were reisolate-negative for the bladder tissue. One mouse was reisolate-negative for the heart and joint tissues. One mouse was reisolate-negative for the heart tissue.