Table 5. List of primers used in this study.

Primer number	Designation	Sequence (5′ – 3′)a
1	pncA 5′ EcoRI A	cggaattcatgGCACTTATTTTAATAGATATAC
2	pncA 3′ XbaI	gctctagaTTATATATTAAGCTTACTTTGGCTG
3	ospC prom 5′ EcoRI	cggaattcTTCTTTTTCATTAATTTGTGCCTCC
4	ospC prom 3′ EcoRI	cggaattcTTAATTTTAGCATATTTGGCTTTGCTTATGTCG
5	pUC18R BSV2	AGCGGATAACAATTTCACACAG
6	pncA prom 3′ seq	ACTGTTAGATACTGGCAAAGTGCC
7	bbk46Fup500	GTTCTTTTATGGAGCAAGCAACTAA
8	bbk46Rup500	CGGAAGCCACAAGAGGCGACAGACACTATCTTAGTACCTCTTCTTAGAATCTG
9	bbk46Fdown500	GGCGAGATCACCAAGGTAGTCGGCAAATAAATAATACTAATCTTAGATAGCTCAGCTTT
10	bbk46Rdown500	CTAGCTTCACTAGTTTCCCTAGA
11	flaBpaadA F	TGTCTGTCGCCTCTTGTG
12	flaBpaadA R	TTATTTGCCGACTACCTTGGTG
13	K465′kpn1fwd	cggggtaccCTTCCAGTGTAGGCTTTAGTTT
14	K463′FLAGrev	TTAtttatcatcatcatctttataatcTGCCTCAACTGCCTTTCTC
15	K465′FLAGfwd	gattataaagatgatgatgataaaTAAAATGCTTCAAAGGAAAATTATGAATGG
16	K463′C-mycSalIrev	acgcgtcgacTTAcagatcttcttcagaaataagtttttgttcATAAGCAGCTTCATATGCTTTATTT
17	K465′PCR3fwd	CGGGGTACCCTTCCAGTGTAG
18	K463′PCR3rev	ACGCGTCGACTTACAGATCTTCTTCAGAAATA
19	Lp3629018F	AGCATTATTTGTACTTCTAGGC
20	Lp3629013R	ACATACTAGACAACAACAAGTC
21	flaBF3	GCATTAACGCTGCTAATCTTAG
22	flaBR3	GCATTAATCTTACCAGAAACTCC
23	recA F	AATAAGGATGAGGATTGGTG
24	recA R	GAACCTCAAGTCTAAGAGATG
25	ospC1 F	ACGGATTCTAATGCGGTTTTACCT
26	ospC1 R	CAATAGCTTTAGCAGCAATTTCATCT
27	flaBp 5′ KpnI	gggggtaccTGTCTGTCGCCTCTTGTGGCT
28	flaBp 3′ BamHI	gggggatccGATTGATAATCATATATCATTCCT
29	bbk46+S 5′ BamHIF	cgggatccATGAATTTAATTGCTAAATTATTTATTTTATCCAC
30	bbk46-S 5′ BamHIF	cgggatcc ATGTGTAACCTATATGATAATCTTGCAGAC
31	bbk46 3′ XhoIR	ccgctcgag TTAATAAGCAGCTTCATATGCTTTATTTAG

^aLowercase indicates all non-B. burgdorferi sequence.