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. 2013 Aug 29;9(8):e1003586. doi: 10.1371/journal.ppat.1003586

Figure 1. Gene structure of SmGluCl-1, SmGluCl-2, SmGluCl-3 and SmGluCl-4.

Figure 1

Gene structures of SmGluCl-1 (Smp_096480), SmGluCl-2 (Smp_015630), SmGluCl-3 (Smp_104890) and SmGluCl-4 (Smp_099500/Smp_176730).A–C. SmGluCl-1, SmGluCl-2 and SmGluCl-3 cloned cDNAs contain all the conserved domains and features characteristic of a full length, functional Cys-loop LGIC subunit. D. Full length SmGluCl-4 cDNA sequence could not be determined. SmGluCl-4 partially cloned cDNA lacks an initiation and termination codon, and does not encode a SP and M4. Contig misassembly could explain the 83,509 bp gap found between the two Cys-loop motifs of SmGluCl-4. Graphical representation of gene structures was performed with the Exon-Intron Graphic Maker version 4 (wormweb.org/exonintron). Scales are shown on the right side. Numbers next to the introns correspond to their size; the introns are not to scale. Smp_015630, Smp_096480, Smp_104890, Smp_099500 and Smp_176730 putative genes were obtained from the Schistosoma mansoni homepage on GeneDB (http://www.genedb.org/Homepage/Smansoni). SmGluCl-1 (1,536 bp; KC861381), SmGluCl-2.1 (1,659 bp; KC861382), SmGluCl-2.2 (1,638 bp; KC861383), SmGluCl-3 (1,545 bp, KC861384) and SmGluCl-4 (1,986 bp, KC861385) genes were cloned and amplified from adult worm cDNA. ECD, extracellular binding domain; ICD, intracellular domain; M1–4, membrane-spanning region 1–4; SP, signal peptide. Grey areas indicate regions that do not correspond to any known functional domains.