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. 2013 Aug 29;9(8):e1003569. doi: 10.1371/journal.ppat.1003569

Figure 4. C. pneumoniae induces IFN-β and IL-10 production in BMDM from B6.C3H-sst1 via TBK1/IKKξ dependent pathway and is independent of MyD88.

Figure 4

BMDM were prepared from B6.C3H-sst1 mice as described in the Methods. Prior to infection with C. pneumoniae (Cp) or treatment with LPS, cells were pretreated with IFN-γ (5 U/ml) and the following inhibitors for 30 min at the indicated concentrations; supernatant was harvested at 24 hpi for assay of IL-10 and IFN-β. A–B: TBK1/IKKξ inhibitor BX795 or vehicle control (DMSO); or the PKR inhibitor 2-aminopurine (2-AP) or vehicle control PBS∶Glacial acetic acid at a ratio of 200∶1 (Glacial AA). C–D: MYD88 inhibitor Pepinh-MYD (Pep-MYD), or Pepinh-control (Pep-Ctr) Significance: *, p≤0.05; **, p≤0.01; ***, p≤0.001 compared to corresponding control. The results are representative of 2 independent experiments.