Figure 3. Flash responses from wild type, Gα_GTP ^G38D+/−, and Gα_GTP ^G38D−/− rods.

Experimental (A, B, C) versus simulated (D, E, F) responses to flashes of increasing intensities from wild type Gα_GTP ^WT+/+ (A, D), heterozygous Gα_GTP ^G38D+/− (B, E) and homozygous Gα_GTP ^G38D−/− (C, F) rods are shown. Experimental data, i.e. published in Moussaif et al. [8] and provided by Marie E. Burns, refer to mice rods exposed to flashes ranging from 5 to 97000 photons µm⁻² (A and B) or from 650 to 94000 photons µm⁻² (C). Simulated responses derive from the model of an amphibian rod stimulated with the same light intensities as in vitro recordings. The pathological Gα_GTP ^G38D+/− and Gα_GTP ^G38D−/− models were generated by changes in the kinetic parameters kP1, kP2 and kRGS1 as described in the text. The dissimilar species justify the time scale difference between in vitro and in silico experiments. The responses were normalized with respect to the maximum photocurrent. G. Normalized simulated light response amplitude is plotted as a function of flash strength. For comparison to in vitro data, see Figure 5B in Moussaif et al. [8]. Flash intensities are the same as in D, E and F.