Table 1. Reactions that were changed in the background of the G38D mutation.

Na	Reaction Equation	Descriptionb	O.P.c	Mind	Maxd	Effecte
1		Intrinsic GαGTP GTPase activity	0.05	×2	0	None
2		Intrinsic GαGTP-PDE GTPase activity	0.033	×2	0	Slowed recovery when = 0
2		Intrinsic GαGTP-PDE- GαGTP GTPase activity	0.033	×2	0	Slowed recovery when = 0
3		Binding of GαGTP to inactive PDE	5.5e-2	×2	×109	Slowed activation, lower sensitivity and recovery with changes >×106
4		Activation of one PDE catalytic subunit	940.7	×2	×104	Slowed activation phase with changes >×103
5		Binding of GαGTP to GαGTP-PDE	1.498e-9	×2	×105	None
6		Activation of the PDE tetramer	21.09	×2	×105	None
7		Binding of RGS9 to GαGTP-PDE	1.57e-7	×2	×15	Slowed recovery phase
7		Binding of RGS9 to GαGTP-PDE-GαGTP	1.57e-7	×2	×15	Slowed recovery phase
8		GAP activity and disruption of the complexes	256.07	×2	×105	Increase in saturating phase for bright flashes with changes >102
8		GAP activity and disruption of the complexes	256.07	×2	×105	Increase in saturating phase for bright flashes with changes >102

^aReaction number, corresponding to that in Figure 1.

^bType of reaction.

^cOriginal value of the parameter governing the reaction kinetics in the mathematical model (s⁻¹) [14].

^dMinimum and maximum reductions the considered parameters were subjected to in the present study, in order to model the mutated conditions; when “Max R” was 0, the parameter was ultimately set to zero.

^eEffect of these reductions.