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. 2013 Aug 29;7(8):e2395. doi: 10.1371/journal.pntd.0002395

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© 2013 Nieuwenhuizen et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) 4E8g recognizes a 40 kDa protein in A. lumbricoides extract (Al) and a 37 kDa protein in A. pegreffii somatic extract (Ap) and excretory-secretory proteins (ES) B) Antigen recognition in different nematode extracts. Ap, Anisakis pegreffii; Al, Ascaris lumbricoides; C, Contracaecum sp.; Hp, Heligosomoides polygyrus; Nb, Nippostrongylus brasiliensis; So, Syphacia obvelata. 4E8g antigen could be successfully purified from both (C) somatic and (D) excretory-secretory extracts of Anisakis by immunoaffinity spin columns and was identified as Anisakis haemoglobin. 1–6 = elution fractions 1–6, FT, flowthrough of spin column, Ap, Anisakis pegreffii, ES, excretory-secretory proteins.