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. 2013 Aug 29;7(8):e2395. doi: 10.1371/journal.pntd.0002395

Figure 7. Monoclonal antibody 4E8g does not recognize mouse haemoglobin.

Figure 7

A) Alignment of sequences of A.pegreffii, A.lumbricoides, human and mouse haemoglobin was carried out using MUSCLE (http://www.ebi.ac.uk/Tools/muscle/index.html). Percentage agreement to consensus sequence is coloured as follows: mid-blue: >80%, light blue: >60%, light grey >40%, white </ = 40%. B) Binding of 4E8g to mouse blood was measured by ELISA. Whole mouse blood was collected in EDTA to prevent coagulation, based on commercially available mouse haemoglobin ELISA protocols. Plates were coated with Anisakis extract (positive control) or mouse blood using concentrations from 0.4 ng/ml to 4 mg/ml and incubated with monoclonal antibody 4E8g to assess binding. Detection was carried out using alkaline phosphatase labelled anti-mouse IgG1 with P-nitrophenylphosphate substrate.