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. Author manuscript; available in PMC: 2014 Sep 15.
Published in final edited form as: Dev Biol. 2013 Jul 11;381(2):482–490. doi: 10.1016/j.ydbio.2013.07.001

Fig. 2. ewg is expressed in all photoreceptors and functions autonomously to specify R8 subtypes.

Fig. 2

(A–E) Ewg (red) is co-expressed with Elav (green), a neuronal marker, in all photoreceptors starting from 3rd instar larval stage and remains in pupal and adult retinas. (A'–E') Ewg (red) channel only. (A) y1w67eye imaginal disc at the 3rd instar stage. Ewg is co-expressed with Elav only in differentiated photoreceptors. Expression of Ewg remains at 0% (B), 50% (C), 75% pupation (D) and throughout adult stages (E). Ewg is visible both in nuclei of outer and R7 photoreceptors (outer layer) as well as in R8 in the lower nuclear layer. (F) Most ewg R8 mutant cells (arrow) in clones marked by GFP (green) express Rh6 (red). (F') Red (Rh6) and blue (Rh5) channel only. Among 60 mutant R8s, 56 expressed Rh6. (G) One ewg mutant R8 (arrow) marked by GFP (green) expresses Rh5 (blue). (G') Red (Rh6) and blue (Rh5) channel only.