Figure 5.
In vivo transfection of SSANs in mouse PECs following i.p. injection at 5 μg pCMV-Luc/mouse. (A) Uptake level of SSANs containing YOYO-1-DNA in PECs as monitored by flow cytometry 4 h post administration. M1 and M2 phases corresponded to negative and positive cells, respectively. (B) Transfection efficiencies of SSANs in PECs. PECs were harvested 4 h after i.p. injection of SSANs and were further cultured on 96-well plates for 20 h before luminescence measurement. LPF2000, PEI, jetPEI, and naked DNA served as controls. Results were indicated as mean ± SD (n=4).