Fig. 2.

Effects of A2 adenosine receptors antagonists on inosine effects in the OVA-induced inflammation. Immunized mice were pre-treated with ZM241385 (ZM) or alloxazine (Al), 30 min before inosine. The treatments were performed 30 min before the challenges. After 24 h, the total leukocyte counting (a) and the differential counting of macrophages (b), lymphocytes, neutrophils and eosinophils (c) were performed in the BALF. B basal group not immunized, challenged or treated; OVA ovalbumin-immunized and challenged mice; V ovalbumin-immunized and challenged mice treated with vehicle used to dilute the antagonists; ZM + Ino ovalbumin-immunized and challenged mice pre-treated with ZM241385 and treated with inosine; Al + Ino ovalbumin-immunized and challenged mice pre-treated with allozaxine and treated with inosine; Inosine ovalbumin-immunized and challenged mice treated with inosine. Each column represents the mean of the values obtained in five to eight animals, and the error bars indicate the SEM. *P < 0.05; **P < 0.01; ***P < 0.001 when compared with vehicle group (V); ^#P < 0.001 when compared with the basal group (B); ⁺P < 0.05; ⁺⁺⁺P < 0.001 when compared with the inosine-treated group