Fig. 6.

a Coronal sections of the hypothalamus at the level of PVN modified from the brain atlas of Paxinos and Watson (2004), showing the center of the most microinjections of ATP (unfilled star) and α,β-meATP dose–response curve (unfilled diamond), L-glu or ATP vs. PPADS (unfilled circle), ATP vs. KYN or PPADS + KYN (unfilled square), ATP vs. AP5 or CNQX (unfilled triangle) protocols. Asterisks represent microinjection sites of all protocols outside of the PVN. b Representative photomicrograph of a bilateral microinjection into the PVN (dark field microscopy). The arrows show the position of the micropipette tip. PaDC paraventricular hypothalamic nucleus, dorsal cap, PaLM paraventricular hypothalamic nucleus, lateral magnocellular part, PaMP paraventricular hypothalamic nucleus, medial parvicellular part, PaV paraventricular hypothalamic nucleus, ventral part, Pe periventricular hypothalamic nucleus, Arc arcuate hypothalamic nucleus, 3 V third ventricle