Fig. 2.

Effects of oxATP on the increased P2X₇ expression induced by ischemic injury. The expressions of P2X₇ mRNA and protein were measured by in situ hybridization (a), immunohistochemistry (b), and Western blotting (d). The bar graphs (c, e) showed the statistical results for expression of P2X₇ mRNA or protein. The relative expression in Western blotting was expressed by the IOD ratio of P2X₇ protein to β-actin. The results showed that the expression levels of P2X₇ mRNA or protein in MI group (n = 8) were significantly higher than those in sham group (n = 8), con+oxATP group (n = 8), and MI+oxATP group (n = 8; p < 0.05). No difference was found among sham group, con+oxATP group, and MI+oxATP group (p > 0.05). Arrows indicate the immunostaining neurons. Scale bars, 20 μm. Results are mean ± SE. *p < 0.05 vs sham group, con+oxATP group, and MI+oxATP group