Fig. 6.

The double-label immunofluorescence of P2X₃ and NeuN or HRP in SCG. a NeuN is the marker of neurons. The image shows that P2X₃ receptor was mainly expressed in SCG neurons. Red signal represents P2X₃ staining with TRITC, and green signal indicates NeuN staining with FITC. Merge represents P2X₃ and NeuN double staining image. Scale bar, 20 μm. b There was double immunofluorescence of P2X₃ and HRP from the cardiac afferent endings to SCG, as tested by the retrograde neuronal labeling. Red signal represents P2X₃ staining with TRITC, and green signal indicates HRP staining with FITC. Merge represents P2X₃ and HRP double staining image. Scale bar, 20 μm. c The bar graphs showed the statistical results for co-expression of P2X₃ and NeuN or HRP in SCG (n = 6 in all groups)