Fig. 10.
a Effect of dithiothreitol (DTT) on adenine binding to rAdeR. Radioligand binding assays were performed either with (10 mM) DTT or without DTT using membrane preparations from infected Sf9 cells with [3H]adenine as a radioligand. IC50 value obtained with DTT—158 ± 16 nM; without DTT—173 ± 34 nM. b Effects of DTT on rAde receptor function. Concentration-dependent inhibition of forskolin-induced cAMP accumulation using adenine as agonist was measured using stably transfected 1321N1 astrocytoma cells without or with DTT pretreatment (10 mM, 2 h at 37 °C). Determined EC50 values: without DTT—13.3 ± 1.8 nM; with DTT pretreatment—18.0 ± 1.5 nM. The determined Emax values were not significantly different with and without DTT pretreatment (unpaired t test, p = 0.573)