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[35S]GTPγS binding studies at wt and mutant rat adenine receptors coexpressed with mammalian G proteins. Adenine-induced effects on [35S]GTPγS binding at the wt rat adenine receptor and its mutants N194A and L201A. The radioligand [35S]GTPγS was used at a concentration of 0.5 nM. The results shown represent means ± SEM from three to five independent experiments performed in triplicates. EC50 and Emax values are listed in Table 2
