Table 1.
Adenine | |||||
---|---|---|---|---|---|
IC50 ± SEM (nM) | Numbera | pb | Fold shiftc | Bmax (pmol/mg of protein) | |
wt | 236 ± 35 | 7 | 2.66 ± 0.54 | ||
F110A | 218 ± 53 | 3 | ns | 0.9 | 1.47 ± 0.41 |
N115A | 689 ± 96 | 4 | *** | 2.9 | 3.01 ± 0.36 |
N173A | 397 ± 66 | 4 | *** | 1.7 | 2.56 ± 0.50 |
F179A | 270 ± 48 | 4 | ns | 1.1 | 2.67 ± 0.63 |
N194A | 186 ± 19 | 4 | ns | 0.8 | 1.36 ± 0.39 |
F195A | 292 ± 31 | 4 | * | 1.2 | 2.19 ± 0.75 |
L201A | 206 ± 49 | 4 | ns | 0.9 | 1.90 ± 0.46 |
H252A | 583 ± 94 | 4 | *** | 2.5 | 2.79 ± 0.30 |
Y268A | 302 ± 33 | 4 | * | 1.3 | 2.74 ± 0.85 |
ns not significant
p > 0.05; *p < 0.05; ***p < 0.001
aNumber of experiments performed in triplicates
bLevel of significance of IC50 value determined at mutant as compared to wild-type receptor calculated by the unpaired t test
cThe shift represents the ratio of IC50 (mutant)/IC50 (wt)