Table 1.

IC₅₀ values determined for adenine in homologous radioligand competition binding assays versus [³H]adenine (10 nM) at the wt and mutant rAdeRs using membrane preparations of infected Sf9 cells. Data are means ± SEM of n independent experiments

	Adenine
	IC50 ± SEM (nM)	Numbera	pb	Fold shiftc	Bmax (pmol/mg of protein)
wt	236 ± 35	7			2.66 ± 0.54
F110A	218 ± 53	3	ns	0.9	1.47 ± 0.41
N115A	689 ± 96	4	***	2.9	3.01 ± 0.36
N173A	397 ± 66	4	***	1.7	2.56 ± 0.50
F179A	270 ± 48	4	ns	1.1	2.67 ± 0.63
N194A	186 ± 19	4	ns	0.8	1.36 ± 0.39
F195A	292 ± 31	4	*	1.2	2.19 ± 0.75
L201A	206 ± 49	4	ns	0.9	1.90 ± 0.46
H252A	583 ± 94	4	***	2.5	2.79 ± 0.30
Y268A	302 ± 33	4	*	1.3	2.74 ± 0.85

ns not significant

p > 0.05; *p < 0.05; ***p < 0.001

^aNumber of experiments performed in triplicates

^bLevel of significance of IC₅₀ value determined at mutant as compared to wild-type receptor calculated by the unpaired t test

^cThe shift represents the ratio of IC₅₀ (mutant)/IC₅₀ (wt)