Figure 1.

Loss of PHD1 Inhibits Mitotic Progression

(A) HeLa cells were treated with the indicated siRNAs, and the cell-cycle profile was determined by flow cytometry. Values represent averages of two different experiments. Error bars indicate ± 1 SD.

(B) Transfection of GFP-PHD1 rescues the mitotic arrest of HeLa cells treated with PHD1 siRNA. HeLa cells were transfected with the indicated siRNAs and plasmids and the mitotic index was determined. Values represent averages of three different experiments (n = 100; error bars indicate ± 1 SD; p values are significant according to the Student’s t test; ^∗∗p < 0.01).

(C) Transfection of GFP-PHD1 partially rescues the prometaphase arrest of HeLa cells treated with PHD1 siRNA. HeLa cells were transfected with the indicated siRNAs and plasmids and the mitotic distribution was determined. Values represent averages of two different experiments (n = 100; error bars indicate ± 1 SD; p values are significant according to the Student’s t test; ^∗∗p < 0.01).

(D) PHD1 depletion disrupts the mitotic spindle. Immunofluorescence images of mitotic cells treated with PHD1 siRNA and stained for α-tubulin (green) and DNA (blue). The scale bar represents 5 μm.

(E) Quantification of spindle morphology phenotypes observed in PHD1-depleted cells.

See also Figure S1.