FIGURE 2.

Hinge domain of ERβ1 is responsible for the interaction with Tip60. A, schematic diagram shows the domains of full-length ERβ1 and different domain-deleted constructs. The c-Myc tag was added to the N terminus of each construct. The strength of interaction between different ERβ1 constructs and Tip60 is represented by “+” and “−” signs. “+++” represents the strongest interaction, and “−” represents no interaction. AF-1, activation function 1; AF-2, activation function 2. B, HEK293 cells were grown in CSS-containing medium and transfected with Tip60 and different domain-deleted ERβ1 constructs. Lysates were immunoprecipitated (IP) with c-Myc antibody. Immunoglobulin IgG was used as the negative control. The immunoprecipitates were immunoblotted (IB) with c-Myc or Tip60 antibody. Asterisks denote the positions of ERβ1 and its mutants.