FIGURE 3.

IL-15 induction in response to poly(I:C) requires Sp1, IKKϵ, and NFκB2. A, B, and E, PBMCs were transfected with siRNA targeting Sp1 (A and B), NFκB2 (E), or a non-targeting control siRNA for 48 h. Cells were then stimulated with P(I:C) for 0, 4, and 8 h as indicated. C, PBMCs were pretreated for 1 h with 1 μm mithramycin A (MMA) or left untreated before stimulation with P(I:C). D, WT, IKKϵ^−/−, and NFκB2^−/− BMDMs were stimulated with P(I:C) for 4, 8, and 24 h or left untreated, as indicated. In each case, RT-PCR analysis of RNA was carried out with primers specific for IL-15 or RANTES, as indicated. Expression is normalized to that of GAPDH and is presented relative to that of untreated controls. Data are the mean of at least three separate experiments with each point assayed in triplicate, with error bars representing S.D.