FIGURE 6.

Suppression of the dominant lethal phenotype of rpoZM6 by the rpoC2112 allele. A, cultures of DH5α bearing pRPZM6 and either plasmid pCL1920 (Vector), pHYD535 (rpoB⁺rpoC⁺), or pRPC1 (rpoB⁺rpoC2112) were spotted at the indicated dilutions on LB agar plates containing 0.02% d-glucose (Glu) and 0.02% l-arabinose (Ara) with the appropriate antibiotic selection. B, sequence context of suppressor mutation in E. coli rpoC. The various domains and the active site sequence (boxed) are schematically represented (adapted from Landick and co-workers (68)).