TABLE 1.
List of strains and plasmids in this study
A brief description of each and their origin are given.
| Name | Description | Source/Ref. |
|---|---|---|
| Strains | ||
| DH5α | fhuA2 Δ(argF-lacZ) U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 | |
| MG1655 | Wild-type K12 | |
| PD1 | MG1655 rpoZ::kan | This work |
| JW3624 | rpoZ::kan allele was sourced from the strain for construction of PD1 | |
| 30 | ||
| Bl21(DE3) | dcm ompT hsd S (rB− mB−) galλ (DE3) | 31 |
| RL916 | E. coli rpoC chromosomally His6-tagged | Gift from R. Landick |
| Plasmids | ||
| pBAD18 | Arabinose-inducible protein expression vector | 32 |
| pHYD3011 | Modified pBAD18 | Gift from J. Gowrishankar |
| pRPZ | rpoZ+ cloned in pHYD3011 | This work |
| pCL1920 | Low copy number vector bearing pSC101 replicon | 40 |
| pHYD535 | Plasmid pCL1920 with 10.2-kb HindIII DNA fragment bearing the rplL+ and the rpoB+C+ genes sourced from plasmid pDJJ12 | Gift from R. Harinarayan; 41 |
| pRPZM6 | rpoZ+ N60D cloned in pHYD3011 | This work |
| pRPZM2 | rpoZ+ D36Y,N60D cloned in pHYD3011 | This work |
| pRPZM15 | rpoZ+ A85T cloned in pHYD3011 | This work |
| pRPZM9 | Silent mutation in rpoZ+ cloned in pHYD3011 | This work |
| pRPC1 | rpoCY457S pHYD535 | This work |
| pET21b | Protein expression vector | Novagen |
| pRPC3 | rpoCY457S cloned in pET21b | This work |
| pRPC2 | rpoC cloned in pET21b | This work |
| pRW308 | For expression of RpoC | 38 |
| pETα | For expression of His6-RpoA | 21 |
| pGETB | For expression of RpoB | 43 |
| pARC8112 | For expression of RpoD | 44 |
| pAR1432 | T7A1 promoter fragment | 49 |