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6C2 hinders the interaction between RTA and ribosome. SPR analysis of ribosome binding to RTA in the presence (A) and absence of the antibody 6C2 (B). The signal obtained from the reference surface is subtracted to avoid the binding of non-specificity. C, table showing the binding kinetic parameters. D, pulldown analysis of ribosome binding to RTA in the presence and absence of the antibody 6C2. Mutants disrupting RTA-6C2 binding retain ribosome binding ability. RU, resonance units.
